precision qscript t reverse transcription kit Search Results


qscript one-step rt-qpcr kit  (Quanta Biosciences)
96
Quanta Biosciences qscript one-step rt-qpcr kit
Qscript One Step Rt Qpcr Kit, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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qscript xlt 1-step rt-pcr kit  (Quanta Biosciences)
95
Quanta Biosciences qscript xlt 1-step rt-pcr kit
Qscript Xlt 1 Step Rt Pcr Kit, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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qscript cdna synthesis kit  (Quanta Biosciences)
97
Quanta Biosciences qscript cdna synthesis kit
Qscript Cdna Synthesis Kit, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdna kit  (Quanta Biosciences)
97
Quanta Biosciences cdna kit
Cdna Kit, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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qscript flex cdna synthesis kit  (Quanta Biosciences)
96
Quanta Biosciences qscript flex cdna synthesis kit
Qscript Flex Cdna Synthesis Kit, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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qscript ultra  (Quanta Biosciences)
93
Quanta Biosciences qscript ultra
Qscript Ultra, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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one step sybr green qrt pcr kit  (Quanta Biosciences)
95
Quanta Biosciences one step sybr green qrt pcr kit
One Step Sybr Green Qrt Pcr Kit, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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qscript xlt cdna supermix kit  (Quanta Biosciences)
96
Quanta Biosciences qscript xlt cdna supermix kit
Qscript Xlt Cdna Supermix Kit, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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quantabio qscript cdna supermix kit  (Avantor)
90
Avantor quantabio qscript cdna supermix kit
Quantabio Qscript Cdna Supermix Kit, supplied by Avantor, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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assays pierce bca protein assay vwr 23227 qiamp viral rna mini kit qiagen 52906 qscript flex cdna kit vwr  (Thermo Fisher)
99
Thermo Fisher assays pierce bca protein assay vwr 23227 qiamp viral rna mini kit qiagen 52906 qscript flex cdna kit vwr
Assays Pierce Bca Protein Assay Vwr 23227 Qiamp Viral Rna Mini Kit Qiagen 52906 Qscript Flex Cdna Kit Vwr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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qscript microrna cdna synthesis kit  (Ribobio co)
90
Ribobio co qscript microrna cdna synthesis kit
miR-137 is a direct target of ZFPM2-AS1. (A) Predicted binding site of miR-137 in ZFPM2-AS1. (B) miR-137 expression in SW480 and HCT116 cells transfected with sh-ZFPM2-AS1. (C) miR-137 expression in SW480 and HCT116 cells following transfection with miR-137 mimics or miR-137 inhibitor. (D) Effects of miR-137 mimics on luciferase activity WT or MUT ZFPM2-AS1 gene constructs. (E) Radioimmunoprecipitation and reverse transcription-quantitative PCR analysis of the binding between ZFPM2-AS1 and miR-137. (F) miR-137 expression in CRC tumor tissues and in adjacent normal tissues. (G) miR-137 expression in CRC cell lines. (H) Correlation between ZFPM2-AS1 and miR-137 expressions in CRC tissues. (I and J) Effects of sh-ZFPM2-AS1 and miR-137 inhibitor on proliferation of SW480 and HCT116 cells. (K) Effects of sh-ZFPM2-AS1 and miR-137 inhibitor on cell migration and invasion of SW480 and HCT116 cells (scale bar, 200 µm). **P<0.01, ***P<0.001 vs. control group. miR, <t>microRNA;</t> CRC, colorectal cancer; sh, short hairpin; NC, negative control; OD, optical density; WT, wild-type; MUT, mutant; Ig, immunoglobulin; Ago2, Argonaute 2.
Qscript Microrna Cdna Synthesis Kit, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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miR-137 is a direct target of ZFPM2-AS1. (A) Predicted binding site of miR-137 in ZFPM2-AS1. (B) miR-137 expression in SW480 and HCT116 cells transfected with sh-ZFPM2-AS1. (C) miR-137 expression in SW480 and HCT116 cells following transfection with miR-137 mimics or miR-137 inhibitor. (D) Effects of miR-137 mimics on luciferase activity WT or MUT ZFPM2-AS1 gene constructs. (E) Radioimmunoprecipitation and reverse transcription-quantitative PCR analysis of the binding between ZFPM2-AS1 and miR-137. (F) miR-137 expression in CRC tumor tissues and in adjacent normal tissues. (G) miR-137 expression in CRC cell lines. (H) Correlation between ZFPM2-AS1 and miR-137 expressions in CRC tissues. (I and J) Effects of sh-ZFPM2-AS1 and miR-137 inhibitor on proliferation of SW480 and HCT116 cells. (K) Effects of sh-ZFPM2-AS1 and miR-137 inhibitor on cell migration and invasion of SW480 and HCT116 cells (scale bar, 200 µm). **P<0.01, ***P<0.001 vs. control group. miR, microRNA; CRC, colorectal cancer; sh, short hairpin; NC, negative control; OD, optical density; WT, wild-type; MUT, mutant; Ig, immunoglobulin; Ago2, Argonaute 2.

Journal: Molecular Medicine Reports

Article Title: Long non-coding RNA ZFPM2-AS1 promotes colorectal cancer progression by sponging miR-137 to regulate TRIM24

doi: 10.3892/mmr.2020.11737

Figure Lengend Snippet: miR-137 is a direct target of ZFPM2-AS1. (A) Predicted binding site of miR-137 in ZFPM2-AS1. (B) miR-137 expression in SW480 and HCT116 cells transfected with sh-ZFPM2-AS1. (C) miR-137 expression in SW480 and HCT116 cells following transfection with miR-137 mimics or miR-137 inhibitor. (D) Effects of miR-137 mimics on luciferase activity WT or MUT ZFPM2-AS1 gene constructs. (E) Radioimmunoprecipitation and reverse transcription-quantitative PCR analysis of the binding between ZFPM2-AS1 and miR-137. (F) miR-137 expression in CRC tumor tissues and in adjacent normal tissues. (G) miR-137 expression in CRC cell lines. (H) Correlation between ZFPM2-AS1 and miR-137 expressions in CRC tissues. (I and J) Effects of sh-ZFPM2-AS1 and miR-137 inhibitor on proliferation of SW480 and HCT116 cells. (K) Effects of sh-ZFPM2-AS1 and miR-137 inhibitor on cell migration and invasion of SW480 and HCT116 cells (scale bar, 200 µm). **P<0.01, ***P<0.001 vs. control group. miR, microRNA; CRC, colorectal cancer; sh, short hairpin; NC, negative control; OD, optical density; WT, wild-type; MUT, mutant; Ig, immunoglobulin; Ago2, Argonaute 2.

Article Snippet: Reverse transcription protocol consisted of 37°C for 15 min, followed by 85°C for 5 sec. To determine miRNA expression levels, cDNA was synthesized using the qScript microRNA cDNA Synthesis kit (Guangzhou RiboBio Co., Ltd.).

Techniques: Binding Assay, Expressing, Transfection, Luciferase, Activity Assay, Construct, Real-time Polymerase Chain Reaction, Migration, Negative Control, Mutagenesis

TRIM24 is a direct target of miR-137. (A) Predicted binding site of miR-137 in TRIM24. (B) TRIM24 mRNA expression in CRC tumor and adjacent normal tissue. (C) TRIM24 mRNA expression in CRC cell lines. (D) TRIM24 mRNA and (E) protein expression following transfection with miR-137 mimics or miR-137 inhibitor in SW480 and HCT116 cells. (F) Effects of miR-137 mimics on luciferase activity of WT or MUT TRIM24 constructs. (G) Correlation between TRIM24 and miR-137 expressions in CRC tissues. (H) TRIM24 mRNA and (I) protein expression following transfection with sh-ZFPM2-AS1 and miR-137 inhibitor in SW480 and HCT116 cells. **P<0.01, ***P<0.001 vs. control group. miR, microRNA; CRC, colorectal cancer; TRIM24, tripartite motif containing 24; sh, short hairpin; NC, negative control; WT, wild-type; MUT, mutant.

Journal: Molecular Medicine Reports

Article Title: Long non-coding RNA ZFPM2-AS1 promotes colorectal cancer progression by sponging miR-137 to regulate TRIM24

doi: 10.3892/mmr.2020.11737

Figure Lengend Snippet: TRIM24 is a direct target of miR-137. (A) Predicted binding site of miR-137 in TRIM24. (B) TRIM24 mRNA expression in CRC tumor and adjacent normal tissue. (C) TRIM24 mRNA expression in CRC cell lines. (D) TRIM24 mRNA and (E) protein expression following transfection with miR-137 mimics or miR-137 inhibitor in SW480 and HCT116 cells. (F) Effects of miR-137 mimics on luciferase activity of WT or MUT TRIM24 constructs. (G) Correlation between TRIM24 and miR-137 expressions in CRC tissues. (H) TRIM24 mRNA and (I) protein expression following transfection with sh-ZFPM2-AS1 and miR-137 inhibitor in SW480 and HCT116 cells. **P<0.01, ***P<0.001 vs. control group. miR, microRNA; CRC, colorectal cancer; TRIM24, tripartite motif containing 24; sh, short hairpin; NC, negative control; WT, wild-type; MUT, mutant.

Article Snippet: Reverse transcription protocol consisted of 37°C for 15 min, followed by 85°C for 5 sec. To determine miRNA expression levels, cDNA was synthesized using the qScript microRNA cDNA Synthesis kit (Guangzhou RiboBio Co., Ltd.).

Techniques: Binding Assay, Expressing, Transfection, Luciferase, Activity Assay, Construct, Negative Control, Mutagenesis